Experimentelle Kinderonkologie

Der Fokus unserer Arbeiten liegt auf der Erforschung der Pathogenese des Neuroblastoms und der Übertragung neuer molekularer Erkenntnisse in die klinische Anwendung. Unser Ziel ist, durch ein besseres Verständnis der Tumorbiologie die Behandlungsmöglichkeiten von Neuroblastom-Patienten zu verbessern.

In Deutschland erkranken jährlich etwa 130 Kinder an einem Neuroblastom. Das Neuroblastom ist ein Tumor, der sich zumeist im Bauch- oder Brustraum aus Zellen des peripheren Nervensystems entwickelt. Die Erkrankung weist äußerst unterschiedliche Verläufe auf: Bei etwa der Hälfte der betroffenen Kinder kommt es zu einem aggressiven Tumorwachstum, das trotz intensiver Behandlung oft zum Tod der Patienten führt. In anderen Fällen hingegen bildet sich das Neuroblastom spontan zurück und die Erkrankung heilt ohne eine belastende Chemotherapie aus. Die genetischen Ursachen und die molekularen Prozesse dieser unterschiedlichen Verlaufsformen sind bis heute nicht komplett verstanden.

Zur umfassenden molekularen Charakterisierung der verschiedenen Verlaufsformen des Neuroblastoms nutzen wir moderne Hochdurchsatz-Technologien, wie z.B. Next Generation Sequencing und Microarray-Profiling. Hierdurch können wir tumorspezifische genetische Veränderungen identifizieren, die Aufschluss über die molekularen Mechanismen von Tumor-Regression und –Progression geben und den klinischen Verlauf der Patienten vorhersagen können. Die funktionelle Relevanz der genetischen Veränderungen prüfen wir in Zellkultur-Experimenten und transgenen Mausmodellen. Ein besonderer Fokus liegt auf der Entwicklung neuer Therapieansätze, die gezielt in krebsspezifische Signalwege des Tumors eingreifen. Die Ergebnisse unserer experimentellen Studien nutzen wir zur Entwicklung klinischer Studien, in denen neue diagnostische und therapeutische Verfahren unter kontrollierten Bedingungen geprüft werden.

Das Team

Dr. rer nat. Justus Ackermann
Dr. rer. nat. Christoph Bartenhagen
Dr. Janina Fischer
Dr. Carolina Sterz
Yvonne Kahlert
Anne Engesser
Andrea Krämer, M.Sc.
Elisabeth Arnz, cand. med.
Christine Stindt, cand. med.
Frederik Sand, cand. med.
Esther Walter, cand. med.

Veröffentlichungen

  1. Peifer M, Hertwig F, Roels F, Dreidax D, Gartlgruber M, Menon R, Krämer A, Roncaioli JL, Sand F, Heuckmann J, Ikram F, Schmidt R, Ackermann S, Engesser A, Kahlert Y, Vogel W, Altmüller J, Nürnberg P, Thierry-Mieg J, Thierry-Mieg D, Mariappan A, Heynck S, Mariotti E, Henrich KO, Glöckner C, Bosco G, Leuschner I, Schweiger MR, Savelyeva L, Watkins SC, Shao C, Bell E, Höfer T, Achter V, Lang U, Theissen J, Volland R, Saadati M, Eggert A, de Wilde B, Berthold F, Peng Z, Zhao C, Shi L, Ortmann M, Büttner R, Perner S, Hero B, Schramm A, Schulte JH, Herrmann C, O’Sullivan RJ, Westermann F, Thomas RK, Fischer M
    Telomerase activation by genomic rearrangements in high-risk neuroblastoma. Nature, in press.
  2. Schramm A, Köster J, Assenov Y, Althoff K, Peifer M, Mahlow E, Odersky A, Beisser D, Ernst C, Henssen AG, Stephan H, Schröder C, Heukamp L, Engesser A, Kahlert Y, Theissen J, Hero B, Roels F, Altmüller J, Nürnberg P, Astrahantseff K, Gloeckner C, De Preter K, Plass C, Lee S, Lode HN, Henrich KO, Gartlgruber M, Speleman F, Schmezer P, Westermann F, Rahmann S*, Fischer M*, Eggert A*, Schulte JH*
    Mutational dynamics between primary and relapse neuroblastomas. Nat Genet, Epub ahead of print; *equal contribution.
  3. Zhang W, Yu Y, Hertwig F, Thierry-Mieg J, Zhang W, Thierry-Mieg D, Wang J, Furlanello C, Devanarayan V, Cheng J, Deng Y, Hero B, Hong H, Jia M, Li L, Lin SM, Nikolsky Y, Oberthuer A, Qing T, Su Z, Volland R, Wang C, Wang MD, Ai J, Albanese D, Asgharzadeh S, Avigad S, Bao W, Bessarabova M, Brilliant MH, Brors B, Chierici M, Chu TM, Zhang J, Grundy RG, He MM, Hebbring S, Kaufman HL, Lababidi S, Lancashire LJ, Li Y, Lu XX, Luo H, Ma X, Ning B, Noguera R, Peifer M, Phan JH, Roels F, Rosswog C, Shao S, Shen J, Theissen J, Tonini GP, Vandesompele J, Wu PY, Xiao W, Xu J, Xu W, Xuan J, Yang Y, Ye Z, Dong Z, Zhang KK, Yin Y, Zhao C, Zheng Y, Wolfinger RD, Shi T, Malkas LH, Berthold F, Wang J, Tong W, Shi L, Peng Z, Fischer M
    Comparison of RNA-seq and microarray-based models for clinical endpoint prediction. Genome Biol, 16(1): 133.
  4. Lindner S, Bachmann HS, Odersky A, Schaefers S, Klein-Hitpass L, Hero B, Fischer M, Eggert A, Schramm A, Schulte JH
    Absence of telomerase reverse transcriptase promoter mutations in neuroblastoma. Biomed Rep, 2015, 3(4): 443-446.
  5. Lau DT, Flemming CL, Gherardi S, Perini G, Oberthuer A, Fischer M, Juraeva D, Brors B, Xue C, Norris MD, Marshall GM, Haber M, Fletcher JI, Ashton LJ
    MYCN amplification confers enhanced folate dependence and methotrexate sensitivity in neuroblastoma. Oncotarget, 6(17): 15510-15523.
  6. Oberthuer A, Juraeva D, Hero B, Volland R, Sterz C, Schmidt R, Faldum A, Kahlert Y, Engesser A, Asgharzadeh S, Seeger R, Ohira M, Nakagawara M, Scaruffi P, Tonini GP, Janoueix-Lerosey I, Delattre O, Schleiermacher G, Vandesompele J, Speleman F, Noguera R, Piqueras M, Bénard J, Valent A, Avigad S, Yaniv I, Grundy RG, Ortmann M, Shao C, Schwab M, Eils R, Simon T, Theissen J, Berthold F, Westermann F, Brors B, Fischer M
    Revised risk estimation and treatment stratification of low- and intermediate-risk neuroblastoma patients by integrating clinical and molecular prognostic markers. Clin Cancer Res, 2015, 21(8): 1904-1915.
  7. Ackermann S, Kocak H, Hero B, Ehemann V, Kahlert Y, Oberthür A, Roels F, Theißen J, Odenthal M, Berthold F, Fischer M
    FOXP1 inhibits cell growth and reduces tumorigenicity of neuroblastoma. BMC Cancer, 2014, 14(1): 840.
  8. Su Z, Fang H, Hong H, Shi L, Zhang W, Zhang W, Zhang Y, Dong Z, Lancashire LJ, Bessarabova M, Yang X, Ning B, Gong B, Meehan J, Xu J, Ge W, Perkins R, Fischer M*, Tong W*
    An investigation of biomarkers derived from legacy microarray data for their utility in the RNA-seq era. Genome Biol, 2014, 15(12): 523; *Corresponding author.
  9. Pandey GK, Mitra S, Subhash S, Hertwig F, Kanduri M, Mishra K, Fransson S, Ganeshram A, Mondal T, Bandaru S, Östensson M, Akyürek LM, Abrahamsson J, Pfeifer S, Larsson E, Shi L, Peng Z, Fischer M, Martinsson T, Hedborg F, Kogner P, Kanduri C
    Neuroblastoma transcriptome sequencing reveals a risk associated long noncoding RNA NBAT-1 that controls tumor progression via regulating cell proliferation and neuronal differentiation. Cancer Cell, 2014, 26(5): 722-737.
  10. Dreidax D, Bannert S, Henrich KO, Schröder C, Bender S, Oakes CC, Lindner S, Schulte J, Duffy D, Schwarzl T, Saadati M, Ehemann V, Benner A, Pfister S, Fischer M, Westermann F
    p19-INK4d inhibits neuroblastoma cell growth, induces differentiation and is hypermethylated and downregulated in MYCN-amplified neuroblastomas. Hum Mol Genet, 2014, 23(25): 6826-6837.
  11. Su Z, Łabaj PP, Li S, Thierry-Mieg J, Thierry-Mieg D, Shi W, Wang C, Schroth GP, Setterquist RA, Thompson JF, Jones WD, Xiao W, Xu W, Jensen RV, Kelly R, Xu J, Conesa A, Furlanello C, Gao H, Hong H, Jafari N, Letovsky S, Liao Y, Lu F, Oakeley EJ, Peng Z, Praul CA, Santoyo-Lopez J, Scherer A, Shi T, Smyth GK, Staedtler F, Sykacek P, Tan XX, Thompson EA, Vandesompele J, Wang MD, Wang J, Wolfinger RD, Zavadil J, Auerbach SS, Bao W, Binder H, Blomquist T, Brilliant MH, Bushel PR, Cai W, Catalano JG, Chang CW, Chen T, Chen G, Chen R, Chierici M, Chu TM, Clevert DA, Deng Y, Derti A, Devanarayan V, Dong Z, Dopazo J, Du T, Fang H, Fang Y, Fasold M, Fernandez A, Fischer M, Furió-Tari P, Fuscoe JC, Gaj S, Gandara J, Gao H, Ge W, Gondo Y, Gong B, Gong M, Gong Z, Green B, Guo C, Guo L, Guo LW, Hadfield J, Hellemans J, Hochreiter S, Jia M, Jian M, Johnson CD, Kay S, Kleinjans J, Lababidi S, Levy S, Li QZ, Li L, Li L, Li P, Li Y, Li H, Li J, Li S, Lin SM, López FJ, Lu X, Luo H, Ma X, Meehan J, Megherbi DB, Mei N, Mu B, Ning B, Pandey A, Pérez-Florido J, Perkins RG, Peters R, Phan JH, Pirooznia M, Qian F, Qing T, Rainbow L, Rocca-Serra P, Sambourg L, Sansone SA, Schwartz S, Shah R, Shen J, Smith TM, Stegle O, Stralis-Pavese N, Stupka E, Suzuki Y, Szkotnicki LT, Tinning M, Tu B, van Delft J, Vela A, Venturini E, Walker SJ, Wan L, Wang W, Wang J, Wang J, Wieben ED, Willey JC, Wu PY, Xuan J, Yang Y, Ye Z, Yin Y, Yu Y, Yuan YC, Zhang J, Zhang KK, Zhang W, Zhang W, Zhang Y, Zhao C, Zheng Y, Zhou Y, Zumbo P, Tong W, Kreil DP, Mason CE, Shi L
    A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium. Nat Biotechnol, 2014, 32(9): 903-914.
  12. Walz S, Lorenzin F, Morton J, Wiese KE, von Eyss B, Herold S, Rycak L, Dumay-Odelot H, Karim S, Bartkuhn M, Roels F, Wuestefeld T, Fischer M, Teichmann M, Zender L, Wei CL, Sansom O, Wolf E, Eilers M
    Activation and repression by oncogenic Myc shape tumour-specific gene expression profiles. Nature, 2014, 511(7510): 483-487.
  13. Theissen J, Oberthuer A, Hombach A, Volland R, Hertwig F, Fischer M, Spitz R, Zapatka M, Brors B, Ortmann M, Simon T, Hero B, Berthold F
    Chromosome 17/17q gain and unaltered profiles in high resolution array-CGH are prognostically informative in neuroblastoma. Gene Chromosome Canc, 2014, 53(8): 639-649.
  14. Fabian J, Lodrini M, Oehme I, Schier M, Thole T, Hielscher T, Kopp-Schneider A, Opitz L, Capper D, von Deimling A, Wiegand I, Milde T, Mahlknecht U, Westermann F, Popanda O, Roels F, Hero B, Berthold F, Fischer M, Kulozik A, Witt O, Deubzer HGRHL1 acts as tumor suppressor in neuroblastoma and is negatively regulated by MYCN and HDAC3. Cancer Res, in press.
  15. Schulte JH, Schulte S, Heukamp LC, Astrahantseff K, Stephan H, Fischer M, Schramm A, Eggert A
    Targeted therapy for neuroblastoma: ALK inhibitors. Klin Padiatr, 2013, 225(6): 303-308. IF: 1.059
  16. Schelhorn SE, Fischer M, Tolosi L, Altmüller J, Nürnberg P, Pfister H, Lengauer T, Berthold F
    Sensitive detection of viral transcripts in human tumor transcriptomes. PLoS Comput Biol, 2013, 9(10): e1003228.
  17. Brockmann M, Poon E, Berry T, Carstensen A, Deubzer HE, Rycak L, Jamin Y, Thway K, Robinson SP, Roels F, Witt O, Fischer M, Chesler L, Eilers M
    Small molecule inhibitors of Aurora-A induce proteasomal degradation of N-Myc in childhood neuroblastoma. Cancer Cell, 2013, 24(1): 75-89.
  18. Lodrini M, Oehme I, Schroeder C, Milde T, Schier M, Kopp-Schneider A, Schulte J, Fischer M, De Preter K, Pattyn F, Castoldi M, Muckenthaler M, Kulozik A, Westermann F, Witt O, Deubzer H
    MYCN and HDAC2 cooperate to repress miR-183 signaling in neuroblastoma. Nucleic Acids Res, 2013, 41(12): 6018-6033.
  19. Aschoff M, Hotz-Wagenblatt A, Glatting KH, Fischer M, Eils R, König R SplicingCompass: differential splicing detection using RNA-Seq data. Bioinformatics, 2013, 29(9): 1141-1148.
  20. Kocak H, Ackermann S, Hero B, Kahlert Y, Oberthuer A, Juraeva D, Roels F, Theissen J, Westermann F, Deubzer H, Ehemann V, Brors B, Odenthal M, Berthold F, Fischer M
    Hox-C9 activates the intrinsic pathway of apoptosis and is associated with spontaneous regression in neuroblastoma. Cell Death Dis, 2013, 4:e586.
  21. Dreidax D, Gogolin S, Schroeder C, Muth D, Brueckner LM, Hess EM, Zapatka M, Theißen J, Fischer M, Ehemann V, Schwab M, Savelyeva L, Westermann F
    Low p14ARF expression in neuroblastoma cells is associated with repressed histone mark status, and enforced expression induces growth arrest and apoptosis. Hum Mol Genet, 2013, 22(9): 1735-1745.
  22. Molenaar J, Domingo-Fernández R, Ebus M, Lindner S, Koster J, Drabek K, Mestdagh P, van Sluis P, Valentijn L, van Nes J, Broekmans M, Haneveld F, Volckmann R, Bray I, Heukamp L, Sprüssel A, Thor T, Kieckbusch K, Klein-Hitpass L, Fischer M, Vandesompele J, Schramm A, van Noesel M, Varesio L, Speleman F, Eggert A, Stallings R, Caron H, Versteeg R, Schulte JH
    LIN28B induces neuroblastoma and enhances MYCN levels via let-7 suppression. Nat Genet, 2012, 44(11): 1199-1206.
  23. Schleiermacher G, Mosseri V, London WB, Maris JM, Brodeur GM, Attiyeh E, Haber M, Khan J, Nakagawara A, Speleman F, Noguera R, Tonini GP, Fischer M, Ambros I, Monclair T, Matthay KK, Ambros P, Cohn SL, Pearson ADJ
    Segmental chromosomal alterations have prognostic impact in neuroblastoma: a report from the INRG project. Br J Cancer, 2012, 107(8): 1418-1422.
  24. Höpker K, Hagmann H, Khurshid S, Chen S, Hasskamp P, Seeger-Nukpezah T, Schilberg K, Heukamp L, Lamkemeyer T, Sos M, Thomas R, Lowery D, Fischer M, Roels F, Liebau MC, Resch U, Kisner T, Röther F, Bartram MP, Müller RU, Fabretti F, Kurschat P, Schumacher B, Medema R, Yaffe MB, Schermer B, Reinhardt HC, Benzing T
    AATF/Che1 acts as a phosphorylation-dependent molecular modulator to repress p53-driven apoptosis. EMBO J, 2012, 31(20): 3961-3975.
  25. Stigliani S, Coco S, Moretti S, Oberthuer A, Fischer M, Theissen J, Gallo F, Garaventa A, Berthold F, Bonassi S, Tonini GP, Scaruffi P.
    Identification of novel integrated genomic and RNA signatures associated with progression of metastatic high-risk neuroblastoma. Neoplasia, 2012, 14(9): 823-832.
  26. Asgharzadeh S, Salo JA, Ji L, Oberthuer A, Fischer M, Berthold F, Hadjidaniel M, Liu CWY, Metelitsa LS, Pique-Regi R, Wakamatsu P, Villablanca JG, Kreissman SG, Matthay KK, Shimada H, London WB, Sposto R, Seeger RC
    Clinical Significance of Tumor-Associated Inflammatory Cells in Metastatic Neuroblastoma. J Clin Oncol, 2012, 30(28): 3525-3532.
  27. Peifer M, Fernández-Cuesta L, Sos ML, George J, Kasper LH, Seidel D, Plenker D, Leenders F, Sun R, Zander T, Menon R, Koker M, Dahmen I, Müller C, Di Cerbo V, Schildhaus HU, Altmüller J, Baessmann I, Becker C, De Wilde B, Vandesompele J, Böhm D, Ansén S, Gabler F, Wilkening I, Heynck S, Heuckmann JM, Lu X, Cibulskis K, Banerji S, Getz G, Park KS, Rauh D, Grütter C, Fischer M, Pasqualucci L, Wright G, Wainer Z, Russell P, Petersen I, Chen Y, Stoelben E, Ludwig C, Schnabel P, Hoffmann H, Muley T, Brockmann M, Engel-Riedel W, Muscarella LA, Fazio VM, Groen H, Timens W, Sietsma H, Thunnissen E, Smit E, Heideman DAM, Snijders PJF, Cappuzzo F, Ligorio C, Damiani S, Field J, Solberg S, Brustugun OT, Lund-Iversen M, Sänger J, Clement JH, Soltermann A, Moch H, Weder W, Solomon B, Soria JC, Validire P, Besse B, Brambilla E, Brambilla C, Lantuejoul S, Lorimier P, Schneider PM, Hallek M, Pao W, Meyerson M, Sage J, Shendure J, Schneider R, Büttner R, Wolf J, Nürnberg P, Perner S, Heukamp LC, Brindle PK, Haas S, Thomas RK
    Small cell lung cancer: identification of relevant mutated genes in a highly mutated genome by integrative genome analyses. Nat Genet, 2012, 44(10): 1104-1110.
  28. Frommolt P, Abdallah AT, Altmüller J, Motameny S, Becker C, Stemshorn K, Fischer M, Freilinger T, Nürnberg P
    Assessing the enrichment performance in targeted resequencing experiments. Hum Mutat, 2012, 33(4): 635-641.
  29. Garcia I, Mayol G, Rios J, Domenech G, Cheung NK, Oberthuer A, Fischer M, Maris JM, Brodeur GM, Hero B, Rodriguez E, Sunol M, Galvan P, de Torres C, Mora J, Lavarino C.
    A three-gene expression signature model for risk stratification of patients with neuroblastoma. Clin Cancer Res, 2012, 18(7): 2012-2023.
  30. Coco S, Theissen J, Scaruffi P, Stigliani S, Moretti S, Oberthuer A, Valdora F, Fischer M, Gallo F, Hero B, Bonassi S, Berthold F, Tonini GP
    Age-dependent accumulation of genomic aberrations and deregulation of cell cycle and telomerase genes in metastatic neuroblastoma. Int J Cancer, 2012, 131(7): 1591-1600.
  31. Schulte JH, Bachmann H, Brockmeyer B, DePreter K, Oberthuer A, Ackermann S, Kahlert Y, Pajtler K, Theissen J, Westermann F, Vandesompele J, Speleman F, Berthold F, Eggert A, Brors B, Hero B, Schramm A, Fischer M
    High ALK receptor tyrosine kinase expression supersedes ALK mutation as a determining factor of an unfavorable phenotype in primary neuroblastoma. Clin Cancer Res, 2011, 17(15): 5082-5092.
  32. Henrich KO, Bauer T, Schulte J, Ehemann V, Deubzer H, Gogolin S, Muth D, Fischer M, Benner A, König R, Schwab M, Westermann F
    CAMTA1, a 1p36 tumor suppressor candidate, inhibits growth and activates differentiation programmes in neuroblastoma cells. Cancer Res, 2011, 71(8): 3142-3151.
  33. Afanasyeva EA, Mestdagh P, Kumps C, Vandesompele J, Ehemann V, Theissen J, Fischer M, Zapatka M, Brors B, Savelyeva L, Sagulenko V, Speleman F, Schwab M, Westermann F
    MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. Cell Death Differ, 2011, 18(6): 974-984.
  34. Ackermann S, Goeser F, Schulte JH, Schramm A, Ehemann V, Eggert A, Hero B, Berthold F, Fischer M
    Polo-like kinase 1 is a therapeutic target in high-risk neuroblastoma. Clin Cancer Res, 2011, 17(4): 731-741.
  35. Shi L, Campbell G, Jones WD, Campagne F, Wen Z, Walker SJ, Su Z, Chu TM, Goodsaid FM, Pusztai L, Shaughnessy JD Jr, Oberthuer A, Thomas RS, Paules RS, Fielden M, Barlogie B, Chen W, Du P, Fischer M, Furlanello C, Gallas BD, Ge X, Megherbi DB, Symmans WF, Wang MD, Zhang J, Bitter H, Brors B, Bushel PR, Bylesjo M, Chen M, Cheng J, Cheng J, Chou J, Davison TS, Delorenzi M, Deng Y, Devanarayan V, Dix DJ, Dopazo J, Dorff KC, Elloumi F, Fan J, Fan S, Fan X, Fang H, Gonzaludo N, Hess KR, Hong H, Huan J, Irizarry RA, Judson R, Juraeva D, Lababidi S, Lambert CG, Li L, Li Y, Li Z, Lin SM, Liu G, Lobenhofer EK, Luo J, Luo W, McCall MN, Nikolsky Y, Pennello GA, Perkins RG, Philip R, Popovici V, Price ND, Qian F, Scherer A, Shi T, Shi W, Sung J, Thierry-Mieg D, Thierry-Mieg J, Thodima V, Trygg J, Vishnuvajjala L, Wang SJ, Wu J, Wu Y, Xie Q, Yousef WA, Zhang L, Zhang X, Zhong S, Zhou Y, Zhu S, Arasappan D, Bao W, Lucas AB, Berthold F, Brennan RJ, Buness A, Catalano JG, Chang C, Chen R, Cheng Y, Cui J, Czika W, Demichelis F, Deng X, Dosymbekov D, Eils R, Feng Y, Fostel J, Fulmer-Smentek S, Fuscoe JC, Gatto L, Ge W, Goldstein DR, Guo L, Halbert DN, Han J, Harris SC, Hatzis C, Herman D, Huang J, Jensen RV, Jiang R, Johnson CD, Jurman G, Kahlert Y, Khuder SA, Kohl M, Li J, Li L, Li M, Li QZ, Li S, Li Z, Liu J, Liu Y, Liu Z, Meng L, Madera M, Martinez-Murillo F, Medina I, Meehan J, Miclaus K, Moffitt RA, Montaner D, Mukherjee P, Mulligan GJ, Neville P, Nikolskaya T, Ning B, Page GP, Parker J, Parry RM, Peng X, Peterson RL, Phan JH, Quanz B, Ren Y, Riccadonna S, Roter AH, Samuelson FW, Schumacher MM, Shambaugh JD, Shi Q, Shippy R, Si S, Smalter A, Sotiriou C, Soukup M, Staedtler F, Steiner G, Stokes TH, Sun Q, Tan PY, Tang R, Tezak Z, Thorn B, Tsyganova M, Turpaz Y, Vega SC, Visintainer R, von Frese J, Wang C, Wang E, Wang J, Wang W, Westermann F, Willey JC, Woods M, Wu S, Xiao N, Xu J, Xu L, Yang L, Zeng X, Zhang J, Zhang L, Zhang M, Zhao C, Puri RK, Scherf U, Tong W, Wolfinger RD; MAQC Consortium.
    The MicroArray Quality Control (MAQC)-II study of common practices for the development and validation of microarray-based predictive models.. Nat Biotechnol, 2010, 28(8): 827-838.
  36. Parry RM, Jones W, Stokes TH, Phan JH, Moffitt RA, Fang H, Shi L, Oberthuer A, Fischer M, Tong W, Wang MD
    k-Nearest neighbor models for microarray gene expression analysis and clinical outcome prediction. Pharmacogenomics J, 2010, 10: 292-309.
  37. Oberthuer A, Juraeva D, Li L, Kahlert Y, Westermann F, Eils R, Berthold F, Shi L, Wolfinger RD, Fischer M, Brors B
    Comparison of performance of one-color and two-color gene-expression analyses in predicting clinical endpoints of neuroblastoma patients. Pharmacogenomics J, 2010, 10: 258-266.
  38. Oberthuer A, Hero B, Berthold F, Juraeva D, Faldum A, Kahlert Y, Asgharzadeh S, Seeger R, Scaruffi P, Tonini GP, Janoueix-Lerosey I, Delattre O, Schleiermacher G, Vandesompele J, Vermeulen J, Speleman F, Noguera R, Piqueras M, Bénard J, Valent A, Avigad S, Yaniv I, Weber A, Christiansen H, Grundy RG, Schardt K, Schwab M, Eils R, Warnat P, Kaderali L, Simon T, DeCarolis B, Theissen J, Westermann F, Brors B, Fischer M
    Prognostic impact of gene expression-based classification for neuroblastoma. J Clin Oncol, 2010, 28(21): 3506-3515.
  39. Fischer M, Berthold F
    The role of complex genomic alterations in neuroblastoma risk estimation. Genome Med, 2010, 2: 31. IF: 5.338
  40. Muth D, Ghazaryan S, Eckerle I, Beckett E, Pöhler C, Batzler J, Beisel C, Gogolin S, Fischer M, Henrich KO, Ehemann V, Gillespie P, Schwab M, Westermann F
    Transcriptional repression of SKP2 is impaired in MYCN-amplified neuroblastoma. Cancer Res, 2010, 70(9): 3791-3802.
  41. De Preter K, Vermeulen J, Brors B, Delattre O, Eggert A, Fischer M, Janoueix-Lerosey I, Lavarino C, Maris J, Mora J, Nakagawara A, Oberthür A, Ohira M, Schleiermacher G, Schramm A, Schulte J, Wang Q, Westermann F, Speleman F, Vandesompele J
    Accurate outcome prediction in neuroblastoma across independent datasets using a multi-gene signature. Clin Cancer Res, 2010, 16(5): 1532-1541.
  42. Fischer M, Bauer T, Oberthür A, Hero B, Theissen J, Ehrich M, Spitz R, Westermann F, Brors B, König R, Berthold F
    Integrated genomic profiling identifies two distinct molecular subtypes with divergent outcome in neuroblastoma with loss of chromosome 11q. Oncogene, 2010, 29(6): 865-875.
  43. Nevo I, Oberthür A, Edri-Botzer L, Sagi-Assif O, Pasmanik-Chor M, Kariv N, Fischer M, Yron I, Witz IP
    Gene-expression-based analysis of local and metastatic neuroblastoma variants reveals a set of genes associated with tumor progression in neuroblastoma patients. Int J Cancer, 2010, 126(7):1570-81.
  44. Harel L, Tcherpakov M, Costa B, Zapatka M, Oberthuer A, Hansford LM, Levy Z, Avigad S, Yaniv I, Shi L, Eils R, Fischer M, Brors B, Kaplan DR, Fainzilber M
    CCM2 mediates death signalling by the TrkA receptor tyrosine kinase. Neuron, 2009, 63(5): 585-591.
  45. Hoene V, Fischer M, Ivanova A, Wallach T, Berthold F, Dame C
    GATA factors in human neuroblastoma: distinctive expression patterns in clinical subtypes. Br J Cancer, 2009, 101(8): 1481-1489.
  46. Vermeulen J, De Preter K, Naranjo A, Vercruysse L, Van Roy N, Hellemans J, Swerts K, Bravo S, Scaruffi P, Tonini GP, De Bernardi B, Noguera R, Piqueras M, Canete A, Castel V, Janoueix-Lerosey I, Delattre O, Schleiermacher G, Michon J, Combaret V, Fischer M, Oberthür A, Ambros P, Beiske K, Benard J, Marques B, Rubie H, Kohler J, Pötschger U, Ladenstein R, Hogarty MD, McGrady P, London WB, Laureys G, Speleman F, Vandesompele J
    Predicting outcomes for children with neuroblastoma using a multigene-expression signature, a retrospective SIOPEN/COG/GPOH study. Lancet Oncol, 2009, 10(7): 663-671.
  47. Eckerle I, Muth D, Batzler J, Henrich KO, Lutz W, Fischer M, Witt O, Schwab M, Westermann F
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Eingereichte Arbeiten

Deubzer HE, Oehme I, Lodrini M, Schulte J, Wuenschel J, Opitz L, Pickert D, Schier MC, Fabian J, Milde T, von Deimling A, Kopp-Schneider A, Ehemann V, Michaelis M, Cinatl J Jr, Schramm A, Fischer M, Schwab M, Koegl M, Eggert A, Kulozik AE, Witt O

HDAC11 mediated transcriptional repression of BMP4 contributes to neuroblastoma malignancy. Sumitted to Cancer Cell.

Oehme I, Linke JP, Boeck BC, Milde T, Lodrini M, Fischer M, Roth W, Yao TP, Kaden S, Groene HJ, Deubzer HE, Witt O

Histone deacetylase 10 controls autophagy in neuroblastoma. Submitted to Int J Cancer.

Westermann F, Dreidax D, Gogolin S, Muth D, Zapatka M, Theissen J, Schulte J, Bannert S, Batzler J, Rizzato C, Ehemann V, Benner A, Henrich KO, Oberthür A, Beissbarth T, Gillespie P, Eggert A, Brors B, Fischer M, Schwab M

Functional synergy between CDK4 and MDM2 in MYCN/c-MYC-driven malignant progression of human neuroblastomas. Submitted to Oncogene.

Campbell G, Davison TS, Chen W, Lababidi S, Pennello GA, Campagne F, Jones WD, Gallas BD, Perkins RG, Tang R, Hatzis C, Wolfinger RD, Pusztai L, Tillinghast G, Hess KR, Johnson CD, Lambert C, Peng X, Wang SJ, Vishnuvajjala RL, Wang MD, Soukup M, Zhang J, Zhang J, Brors B, Kohl M, Popovici V, Scherer A, Thomas RS, Fielden M, Fischer M, Paules RS, Shaughnessy Jr. J, Oberthuer A, Bylesjo M, Trygg J, Barlogie B, Juraeva D, Eils R, Shi L

Peer review process for microarray classifiers: statistical issues in the MAQC-II project. Submitted to BMC Bioinformatics.

Chambwe N, Dorff K, Cortes R, Deng X, Srdanovic M, Mukherjee P, Chu TM, Neville P, Tan PY, Fischer M, Brors B, Symmans F, Fielden M, Shaughnessy Jr. J, Barlogie B, Thomas R, Lobenhofer E, Paules R, Oberthuer A, Mohri M, Wolfinger R, Demichelis F, Campagne F

Calibration of cross-validation performance estimates improves biomarker model selection in large p small n microarray datasets. Submitted to Nat Biotechnol.

Lababidi S, Zeeberg BR, Pennello G, Pusztai L, Symmans F, Shaughnessy Jr. J, Crowley J, Barlogie B, Fischer M, Oberthuer A, Campbell G, Shi L

Comparison of genomic classifier with clinical variable-based predictors in the MAQC-II project. Submitted to Nat Biotechnol.



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