Experimental Neonatology

Experimentelle Neonatologie

Our research focusses on multifactorial aspects of development that contribute to children’s disorders. Various risk factors e.g. malnutrition, inflammation or prematurity could severely impair children’s life and we now want to unravel the underlying key mechanisms to understand how changes in development can result in children’s diseases. Most likely environmental changes during a critical window of development can induce long term modifications that impair children’s health. We hypothesize that these can cause epigenetic, proteomic and metabolic modifications to alter the communication of cells with the surrounding extracellular matrix (ECM), to impair tissue stability and function and finally to affect childrens health.

In our current research we want to understand how alterations in microRNAs, extracellular matrix proteins and mitochondria function affect cell-matrix interactions to destabilize organ development and function during fetal, neonatal and adolescent life. This research should contribute to the development of new diagnostic tools and therapeutic strategies for children’s disease.

Current Projects

  • Mitochondria dysfunction in the developing cartilage
  • Posttranscriptional regulation of tissue development, homeostasis and disease
  • ECM-Immune interactions
  • Annexins in health and disease

The Team

Univ. Prof. Dr. rer. nat. Bent Brachvogel, leader
Telefon +49 221 478-6996
E-Mail bent.brachvogel@uni-koeln.de

Markus Auler
Björn Bluhm
Inga von Bomhard
Dr. rer. nat. Julia Etich
Christian Frie
Veronika Georgieva
Charlotte Gerards
Tatjana Holzer
Probst Kristina


  1. Scholz P, Auler M, Brachvogel B, Benzing T, Mallman P, Streichert T, Klatt AR. Detection of multiple annexin autoantibodies in a patient with recurrent          miscarriages, fulminant stroke and seronegative antiphospholipid syndrome.    Biochemia Medica in press
  2. Kreft S, Klatt AR, Straßburger J, Pöschl E, Flower RJ,  Eming S, Reutelings­perger C, Brisson A, Brachvogel B. Skin wound repair is not altered in the absence of endogenous AnxA1 or AnxA5, but pharmacological concentrations of AnxA4 and AnxA5 inhibit wound hemostasis. Cells Tissues Organs. 2016;201(4):287-98.
  3. Steiger j, Stephan a, Inkeles MS, RealegenoS, Bruns H, Kröll P, Castro-Kroner J, Sommer A, Batinica M, Pitzler L, Kalscheuer R, Hartmann P, Plum G, Stenger S, Pellegrini M, Brachvogel B, Modlin RL, Fabri M. Imatinib triggers phagolysosome acidification and antimicrobial activity against 2 BCG in glucocorticoid-treated human macrophages. J Immunol. 2016 Jul 1;197(1):222-32.
  4. Pitzler L, Auler  M, Probst K, Frie C, Bergmeier V, Holzer T, Belluoccio D, Van Den Bergen J, Etich J, Ehlen H,  Zhou Z, Bielke W, Pöschl E, Paulsson M, Brachvogel B.  miR-126-3p promotes matrix-dependent perivascular cell attachment, migration and intercellular interaction. Stem cells 2016 May;34(5):1297-309
  5. Zhou Z, Pausch F, Schlötzer-Schrehardt U, Brachvogel B, Pöschl E. Induction of initial steps of angiogenic differentiation and maturation of endothelial cells by pericytes in vitro and the role of collagen IV. Histochem Cell Biol. 2016 Jan 9
  6. Etich J, Brachvogel B. Role of cartilage extracellular matrix for the development and function of the immune system. Z Rheumatol. 2015 Oct;74(8):711-3
  7. Etich J, Holzer T, Pitzler L, Bluhm B, Brachvogel B. In press miR-26a modulates extracellular matrix homeostasis in cartilage. Matrix Biol 2015 Apr;43:27-34.
  8. Brachs S, Turqueti-Neves  A, Stein M, Reimer D, Brachvogel B, Bösl M, Winkler T, Voehringer D, Jäck HMJ, Mielenz D. Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity. Eur J Immunol. (2014) Nov;44(11):3206-19
  9. Brachvogel B, Zaucke F, Dave K, Norris EL, Stermann J, Dayakli M, Koch M, Gorman JJ, Bateman JF, Wilson R. Comparative proteomic analysis of normal and Collagen IX null mouse cartilage reveals altered extracellular matrix composition     and novel components of the collagen IX interactome. J Biol Chem. (2013) May 10;288(19):13481-92
  10. Grskovic I, Kutsch A, Frie C, Groma G, Stermann J, Schlötzer-Schrehardt U, Niehoff  A, Moss SE, Rosenbaum S, Pöschl E, Chmielewski M, Rappl G, Abken H, Bateman    JF, Cheah KS, Paulsson M,Brachvogel B Depletion of annexin A5, annexin A6, and collagen X causes no gross changes in matrix vesicle-mediated mineralization, but lack of collagen X affects hematopoiesis and the Th1/Th2 response. J Bone Miner Res. 2012 Nov;27(11):2399-412.
  11. Etich J, Bergmeier V, Frie C, Kreft S, Bengestrate L, Eming S, Mauch C, Eckes B, Lund FE, Rappl G, Abken H, Paulsson M, Brachvogel B. PECAM1+/ Sca1+/CD38+ vascular cells promote wound repair in skin. PLoS One. (2013);8(1):e53262.
  12. Ueki H, NishimuraY, Rieanrakwong D, YonezawaT, Kurusu S, Hasegawa Y, Brachvogel B, Pöschl E and Kawaminami M. Loss of maternal annexin A5 causes placental thrombosis and foetal loss. Sci Rep. (2012);2:827.
  13. Grskovic I, Kutsch A, Frie C, Groma G, Stermann J, Schlötzer-Schrehardt U,  Niehoff A, Moss SE, Pöschl E, Bateman JF, Cheah KSE, Paulsson M, Brachvogel B. Depletion of annexin A5, annexin A6, and collagen X causes no gross changes in matrix vesicle-mediated mineralization, but lack of collagen X affects hematopoiesis and the Th1/Th2 response. J Bone Miner Res (2012) Nov;27(11):2399-412
  14. Willenborg S, van Loo G, Knipper J, Krieg T, Haase I, Brachvogel B, Nagy A, Ferrara N, Pasparakis M, Eming S. CCR2 recruits an inflammatory macrophage subpopulation critical for angiogenesis in tissue repair. Blood (2012) Jul 19;120(3):613-25
  15. Groma G, Xin W, Grskovic I, Niehoff A, Brachvogel B, Paulsson M, Zaucke F. Abnormal bone formation in COMP and matrilin-3 double deficient mice caused by increased TIMP-3 deposition and delayed aggrecan degradation. Arthritis Rheum (2012) Aug;64(8):2644-54
  16. Wilson R, Norris EL, Brachvogel B, Angelucci C, Zivkovic S, Gordon L, Bernardo BC, Stermann J, Sekiguchi K, Gorman JJ, Bateman JF. Changes in the chondrocyte and extracellular matrix proteome during postnatal mouse cartilage development. Mol Cell Proteomics. (2012) Jan;11(1): M111.014159
  17. Groma G, Grskovic I, Schael S, Ehlen HW, Wagener R, Fosang A, Aszodi A, Paulsson M, Brachvogel B, Zaucke F. Matrilin-4 is processed by ADAMTS-5 in late golgi vesicles present in growth plate chondrocytes of defined differentiation state. Matrix Biol. (2011) May;30(4):275-80
  18. Bouter A, Gounou C, Bérat R, Pöschl E, Brachvogel B and Brisson AR. Assembly of a 2D array of annexin-A5 on the torn cell membrane prevents wound expansion and promotes membrane repair. Nature Commun. (2011);2:270
  19. Rosenbaum S., Kreft S., Grskovic I., Etich J., Frie C., Stermann J., Frey B., Mielenz D., Pöschl E., Paulsson M., Gaipl U., Brachvogel B.. Identification of novel opsonins (annexins) for the phagocytosis signal phosphatidylserine and definition of their cell death recognition motif. J Biol Chem (2011) Feb 18;286(7):5708-16
  20. Eming S., Krieger A., Bruckner-Tuderman L., Kreft S., Brachvogel B.,  Krieg T., Koch M., Fox JW. Differential proteomic analysis distinguishes tissue repair biomarker signatures in wound exudates obtained from normal healing and chronic wounds. J Proteome Res. (2010) Sep 3;9(9):4758-66
  21. Belluoccio D, Etich J, Rosenbaum S, Frie C, Grskovic I, Stermann J, Vogel S, von der Mark K, Bateman JF, Brachvogel B. Sorting of growth plate chondrocytes allows the isolation and characterisation of cells of a defined differentiation status. J Bone Miner Res (2010) Jun;25(6):1267-81
  22. Belluoccio D, Grskovic I, Niehoff A, Schlötzer-Schrehardt U, Rosenbaum S, Etich J, Frie C, Pausch F, Moss SE, Pöschl E, Bateman JF, Brachvogel B. Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization. J Bone Miner Res. (2010) Jan;25(1):141-53.
  23. Allen JM, Zamurs L, Brachvogel B, Schlötzer-Schrehardt U, Hansen U, Lamande SR, Rowley L, Fitzgerald J, Bateman JF. Mice lacking the extracellular matrix protein warp develop normally but have compromised peripheral nerve structure and function. J Biol. Chem. 284, 12020-30 (2009)
  24. Cameron TL, Belluoccio D, Farlie PG, Brachvogel B, Bateman JFGlobal Comparative transcriptome analysis of cartilage formation in vivo. BMC Dev Biol. 9, 20 (2009) 17 pages
  25. Frey B, Munoz LE, Pausch F, Sieber R, Franz S, Brachvogel B, Pöschl E, Schneider H, Rödel F, Sauer R, Fietkau R, Herrmann M, Gaipl, USThe immune reaction against allogeneic necrotic cells is reduced in AnnexinA5 knock out mice whose macrophages display an anti-inflammatory phenotype. J Cell Mol Med. Jun 20. (2008)
  26. Allen, J.M., Brachvogel B, Farlie, P.G., Fitzgerald, J., and Bateman, J.F. Expression of WARP during mouse embryonic development Matrix Biol. 27, 295-305 (2008)
  27. Eming, S., Brachvogel, B., Odorisio, T. and Koch, M. Regulation of angiogenesis: wound healing as a model. Prog. Histochem. Cytochem. 42, 115-170 (2007)
  28. Mittag, J., Oehr, W., Heuer, H., Hämäläinen, T., Brachvogel, B., Pöschl, E. and Bauer, K. Expression and Thyroid Hormone Regulation of Annexins in the Anterior Pituitary. J. Endocrinol. 195, 385-392 (2007)
  29. Brachvogel, B., Pausch, F., Farlie, P., Gaipl, U., Etich, J., Zhou, Z., Cameron, T., Von Der Mark, K., Bateman, J., and Pöschl, E. Isolated Anxa5+/Sca-1+ Perivascular Cells From Mouse Meningeal Vasculature Retain Their Perivascular Phenotype In Vitro and In Vivo. Exp. Cell Res. 313, 2730-43 (2007)
  30. Gaipl, U., Munoz, L., Roedel, M., Pausch, F., Frey, B., Brachvogel, B., Von Der Mark, K., and Pöschl, E. Modulation of the immune system by dying cells and the phosphatidylserine-ligand annexin A5. Autoimmunity 40, 254-259 (2007)
  31. Munoz, L. E., Frey, B., Pausch, F., Baum, W., Mueller, R., Brachvogel, B., Pöschl,E., Rodel, F., von der Mark, K., Herrmann, M., and Gaipl, U. S. The role of annexin A5 in the modulation of the immune response against dying and dead cells. Curr. Med. Chem. 14, 271-277 (2007)
  32. Munoz, L. E., Franz, S., Pausch, F., Furnrohr, B., Sheriff, A., Vogt, B., Kern, P. M., Baum, W., Stach, C., von Laer, D., Brachvogel, B., Pöschl, E., Herrmann, M., and Gaipl, U. S. The influence on the immunomodulatory effects of dying and dead cells of Annexin V. J Leukoc Biol., 81, 6-14 (2006)
  33. B. Brachvogel, H. Moch, F. Pausch, U. Schlötzer-Schrehardt, C. Hofmann, R. Hallmann, K. von der Mark, E. Pöschl. Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages. Development 132, 2657-68 (2005)
  34. E. Pöschl , U. Schlotzer-Schrehardt, B. Brachvogel, K. Saito , Y. Ninomiya, U. Mayer Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. Development 131, 1619-28 (2004)
  35. B. Brachvogel, H.Welzel, H. Moch, J. Dikschas, K. von der Mark, C. Hofmann and E. Pöschl. Annexin A5 is not essential for skeletal development. Mol Cell Biol. 23, 2907-13 (2003)
  36. B. Brachvogel, D. Reichenberg, S. Beyer, K. von der Mark and W. Bielke. Molecular cloning and expression analysis of a novel member of the ADAM-family. Gene 288, 203-10 (2002)
  37. B. Brachvogel, H.Welzel, H. Moch, K. von der Mark, C. Hofmann and E. Pöschl Sequential expression of annexin A5 in the vasculature and skeletal elements during mouse development. Mech Dev. 109, 389-93 (2001)
  38. B. Dreier, S. Fritz, G. Volkert, B. Brachvogel, F. Lottspeich, GH. Fey. A 32-kDa proteolytic fragment of transcription factor Stat3 is capable of specific DNA binding. J Biol Chem. 272, 22118-24 (1997)
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